dsRNA Primer Design Tool

Design and optimize dsRNA primers with off-target species analysis

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Upload Gene Sequences

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Drag and drop FASTA files here, or

Supported: .fasta, .fa, .fas, .txt, .seq (max 50MB)

OR

Analysis Parameters

315-500 bp recommended
For off-target alignment
Number of primers to output

Off-Target Species Selection

Select which species to check for off-target effects. Only indexed species are available.

Target Region Selection

Select which part of the gene to design primers from. Provide coordinates if known.

Select your target pest to score how well each dsRNA candidate covers its transcriptome.

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